Purification of latent phenolase from broad-bean (Vicia faba) leaves
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چکیده
منابع مشابه
Rhizobium alters inducible defenses in broad bean, Vicia faba
Conversion of inorganic nitrogen by mutualistic nitrogen-fixing bacteria is essential for plant growth and reproduction, as well as the development of chemical and mechanical defenses. It is unclear, however, how these bacteria alter cooccurring symbioses at higher trophic levels; e.g., extrafloral nectary (EFN) induction, in response to herbivory, to attract defensive mutualists. We hypothesiz...
متن کاملOxidative Stress and Dna Damage in Broad Bean (vicia Faba
6300 words in the text 6325 This ET&C Paper in Press manuscript is in its original unedited form and has not been copyedited or formatted for final production. This manuscript is fully citable. ©2008 Society of Environmental Toxicology and Chemistry (SETAC). This ET&C Paper in Press manuscript is in its original unedited form and has not been copyedited or formatted for final production. This m...
متن کاملBroad bean (Vicia faba) consumption and Parkinson's disease.
In 1913, Guggenheim identified L-DOPA in the seedlings, pods, and beans of the broad bean, Vicia faba (VF). Since then, anecdotal cases of symptomatic improvement after VF consumption have been described in patients with PD. In the present study, five healthy volunteers and six PD patients (mean age, 63.5 years; mean disease duration, 13 years; stage III, Hoehn-Yahr scale) ate 250 g cooked VF a...
متن کاملSubtilisin Inhibitor from Seeds of Broad Bean (vicia Faba); Purification, Amino Acid Sequence and Specificity of Inhibition
A potent inhibitor of microbial serine proteases, including subtilisin, has been purified 1100-fold from seeds of broad bean (Vicia faba, cv. Kleine Thtiringer). Chymotrypsin and trypsin were not inhibited, but a weak "temporary" inhibition of pancreas elastase was observed. The preparation of pure inhibitor contained one major molecular form with a blocked N-terminal (MW 10,000, pl 4.8) and a ...
متن کاملPurification and preliminary characterization of mitochondrial complex I (NADH: ubiquinone reductase) from broad bean (Vicia faba L.).
NADH:ubiquinone reductase (EC 1.6.19.3), or complex I, was isolated from broad bean (Vicia faba L.) mitochondria. Osmotic shock and sequential treatment with 0.2% (v/v) Triton X-100 and 0.5% (w/v) [3-cholamidopropyl)dimethylammonio]-1-propanesulfate (CHAPS) removed all other NADH dehydrogenase activities. Complex I was solubilized in the presence of 4% Triton X-100 and then purified by sucrose-...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1961
ISSN: 0306-3283
DOI: 10.1042/bj0790514